GC B-cells feature a predominantly hypomethylated DNA methylation signature. (A) Unsupervised hierarchical clustering using the Ward method was performed on all probesets and accurately segregated NB cells from GC B cells (GCB). (B) Principal component analysis of methylation values for NBs and GCBs. The first and second principal components separate NBs from GCBs, underscoring the overall differences in methylation patterning. (C) A signature of differentially methylated genes in GC B-cells versus NB cells based on P < .01 (moderated t test with BH correction) and methylation difference of ∼ 40% was identified and included 235 genes. A heatmap representation allows visualization of the finding that the majority of differentially methylated genes are hypomethylated in GC B cells