Figure 7
Effects of recombinant Notch ligands Jagged-1/-2, DLL-1/-3 and -4 on the survival of B-ALL cells. (A) B-ALL cells were cultured alone and (B) cocultured with hBM-MSCs for 3 days (10:1 ratio) in absence and presence of recombinant Notch ligands Jagged-1/-2, DLL-1/-3 and -4. DLL indicates Delta-like ligand; and Jag, Jagged. Percentage of viable B-ALL cells cocultured with hBM-MSCs in Transwell conditions. (C) B-ALL cells were cocultured with hBM-MSCs (10:1 ratio) in Transwell conditions in absence or presence of anti-Notch-1/-3 and -4 Abs and GSI XII for 3 days. Percentage of viable B-ALL (CD19+) cells was obtained by using flow cytometry. Statistical analysis was done using 1-way ANOVA, Holm-Sidak test. *P < .05. Data were represented as the mean ± SD of 10 independent experiments. DLL indicates Delta-like ligand; and Jag, Jagged ligand.

Effects of recombinant Notch ligands Jagged-1/-2, DLL-1/-3 and -4 on the survival of B-ALL cells. (A) B-ALL cells were cultured alone and (B) cocultured with hBM-MSCs for 3 days (10:1 ratio) in absence and presence of recombinant Notch ligands Jagged-1/-2, DLL-1/-3 and -4. DLL indicates Delta-like ligand; and Jag, Jagged. Percentage of viable B-ALL cells cocultured with hBM-MSCs in Transwell conditions. (C) B-ALL cells were cocultured with hBM-MSCs (10:1 ratio) in Transwell conditions in absence or presence of anti-Notch-1/-3 and -4 Abs and GSI XII for 3 days. Percentage of viable B-ALL (CD19+) cells was obtained by using flow cytometry. Statistical analysis was done using 1-way ANOVA, Holm-Sidak test. *P < .05. Data were represented as the mean ± SD of 10 independent experiments. DLL indicates Delta-like ligand; and Jag, Jagged ligand.

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