Generation of LMP2A/λ-MYC/DN Egr transgenic mice. RNA was isolated from cervical lymph node tumor cells from tumor-burdened transgenic mice of the indicated genotypes or purified B cells from wild-type and DN Egr transgenic mice. Real-time RT-PCR analysis was performed using primers specific for DN Egr (A), CD44 (B), and Icam-1 (C) as well as the housekeeping gene Gapdh. The inset in panel A represents RT-PCR products electrophoresed on 2% agarose gel from 1 experiment. unstim. indicates unstimulated B cells from wild-type and DN Egr mice. All RT-PCR data represent the mean ± SE from 3 independent experiments. The differences in gene expression of all groups were analyzed by 1-way ANOVA and Dunnett multiple comparison tests using wild-type as a control group. ***P < .01. (D) Kaplan-Meier curve shows percentage survival of each transgenic line, LMP2A/λ-MYC/DN Egr: n = 17, LMP2A/λ-MYC: n = 19, λ-MYC/DN Egr: n = 14, λ-MYC: n = 16. Mice were sacrificed when cervical lymph node tumors were observed, at which point the animals could generally survive for a maximum of 7 days.