Histomorphometric scoring of hind limb bones adjacent to tumors in mice treated with a peritumoral dose of BIO or vehicle (DMSO). (A) Example of sections used for the histomorphometric scoring, demonstrating tibial and femoral destruction in untreated but INA6-engrafted knees. Note destruction of the bone at the articular surfaces of the knee (arrowed) and disruption of the trabecular structures that is less apparent in the BIO-treated animals (asterisk). (B) Static histomorphometric assays of peritumoral bone tissue in INA6 bearing mice locally treated with BIO or with vehicle (DMSO). The bone area (B. Area), marrow star volume (MSV), trabecular thickness (Tb. Th.), trabecular star volume (TSV), bone perimeter (B. Perim), and percentage of bone/total tissue (frac × 100) are shown. Bars represent mean (± SEM) for n = 4-5 samples. *P < .05 with Student t test. Where values for P were borderline significant, they are given on the plot. Experiment was performed twice.