American Society of Hematology

Figure 6

$Figure 6. Nutlin-3a abrogates MSC-mediated resistance to FI-700 in primary AML cells. Fifteen FLT3 mutant samples and 15 FLT3 wild-type samples were treated for 72 hours with 800nM FI-700 in the presence or absence of MSCs. Annexin V–positive fractions were determined. MSCs were either Nutlin-3a–pretreated or untreated. In each patient sample, the percentage of annexin V–positive cells in control medium (spontaneous apoptosis) was set as “0” (control) and the extent of apoptosis was expressed as percent changes from this baseline. Results are expressed as mean ± SEM. Asterisk (*) indicates significance at P < .05.$

Nutlin-3a abrogates MSC-mediated resistance to FI-700 in primary AML cells. Fifteen FLT3 mutant samples and 15 FLT3 wild-type samples were treated for 72 hours with 800nM FI-700 in the presence or absence of MSCs. Annexin V–positive fractions were determined. MSCs were either Nutlin-3a–pretreated or untreated. In each patient sample, the percentage of annexin V–positive cells in control medium (spontaneous apoptosis) was set as “0” (control) and the extent of apoptosis was expressed as percent changes from this baseline. Results are expressed as mean ± SEM. Asterisk (*) indicates significance at P < .05.

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