Transgenic mice of subline E express human F8 mRNA in a variety of different organs but express a hemophilic phenotype. (A) Expression of F8 mRNA in transgenic male (▴) and female (●) mice compared with the expression of murine F8 mRNA in wild-type C57BL/6 male (▵) and female mice (○). Total RNA was isolated from liver (li), brain (br), reproductive organs (ro), kidneys (ki), bone marrow (bm), muscle (mu), heart (he), thymus (th), lymph nodes (ln), spleen (sp), and lungs (lu) and reverse transcribed into cDNA. Expression of human and murine F8 mRNA was analyzed in relation to murine β-actin by TaqMan real-time quantitative PCR. Each data point represents results obtained from an individual mouse. (B) Blood loss after tail cut was determined over a period of 60 minutes using the tail-cut model.³⁵  Presented are box plots representing the results obtained with groups of 16 mice. Compared are transgenic mice expressing the human F8 cDNA (huF8), conventional hemophilic E17 F8-knockout mice (E17 F8 ko), and wild-type mice (C57BL/6). ns indicates that the difference was not significant. *P < .001.