Figure (shown in more detail in Figure 1B in Ahmadi et al1) shows 58α-β- T lymphoma cells mock-transduced or transduced with WT1-TCR, WT1-TCR+control-GFP, or WT1-TCR+CD3-GFP, then stained with antibodies against CD3, Vβ2.1, or with HLA-A2/pWT126 tetramer. The numbers in the panels in TCR+CD3-GFP–transduced cells indicate the fold increase in the level of CD3 or TCR expression (measured by mean fluorescence intensity) in cells expressing TCR+CD3-GFP (Q2) compared to the cells expressing TCR only (Q1). No increase in CD3 or TCR expression was seen in cells transduced with TCR+control-GFP.

Figure (shown in more detail in Figure 1B in Ahmadi et al) shows 58α-β- T lymphoma cells mock-transduced or transduced with WT1-TCR, WT1-TCR+control-GFP, or WT1-TCR+CD3-GFP, then stained with antibodies against CD3, Vβ2.1, or with HLA-A2/pWT126 tetramer. The numbers in the panels in TCR+CD3-GFP–transduced cells indicate the fold increase in the level of CD3 or TCR expression (measured by mean fluorescence intensity) in cells expressing TCR+CD3-GFP (Q2) compared to the cells expressing TCR only (Q1). No increase in CD3 or TCR expression was seen in cells transduced with TCR+control-GFP.

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