ZAP-70 enhances CCR7 up-regulation after BCR activation. (A) Expression of CCR7, CXCR3, CXCR4, CXCR5, CD44, CD49d, and CD62L was assessed by flow cytometry in Ramos transfectants before and after stimulation for 16 hours with 5 μg/mL F(ab′)2 anti-IgM. Left panel: MFIR (± SEM) of expression before and after IgM stimulation. Right panel: Change of expression relative to unstimulated cells in Ramos GFP and Ramos GFP-ZAP-70. *P < .05. (B) Expression of CCR7, CXCR3, CXCR4, CXCR5, CD44, CD49d, and CD62L was assessed by flow cytometry in Raji cells with or without ZAP-70 expression. (C) ZAP-70, CCR7, CXCR4, CXCR5, CD44, CD49d, and CD62L expression was assessed by flow cytometry in peripheral blood mononuclear cells obtained from 40 patients with CLL. The expression of ZAP-70 in CD3+ gated T cells was used as internal control and allowed for the measurement of expression of chemokine receptors and adhesion molecules in CD19+/CD5+ gated CLL cells with high (black) or low (gray) ZAP-70 expression. (D) Expression of CCR7, CXCR4, CXCR5, CD44, CD49d, and CD62L was significantly higher in CLL cells with high ZAP-70 expression within the same patient.
