Reduced erythroid and B lymphocytes in the bone marrow, spleens, and peripheral blood of mice receiving Hspa9-shRNA–transduced progenitor cells. (A) The percentage of donor (Ly5.1⁺)–transduced (YFP⁺) cells was measured in the bone marrow, spleens, and peripheral blood of recipient mice up to 8 weeks after transplantation (n = 8-9). (B) Mice were given 2 intraperitoneal injections of BrdU 12 and 24 hours prior to isolating YFP⁺ bone marrow cells and staining them for BrdU incorporation as a measure of cells in the S phase of the cell cycle. (n = 4). (C-D) The contribution of YFP⁺ erythroid precursors was measured in the bone marrow, spleens, and peripheral blood of recipient mice up to 8 weeks after transplantation (n = 8-9). (E-G) The contribution of YFP⁺ B cells, T cells, neutrophils, and monocytes was measured in the bone marrow, spleens, and peripheral blood of recipient mice using flow cytometry up to 8 weeks after transplantation (n = 8-9). All data represent the means ± SD.