Sequestration of Ly49A or Ly49G2 increases the response of iIELs. (A) iIELs from B6 mice were incubated with anti-Ly49A mAb (JR9–318; ) or an isotype-matched control mAb (■). Unbound mAb was removed before stimulation with plate-bound anti-CD3 mAb. MIP-1α production by Ly49A or Ly49G2 single-positive iIELs is shown relative to iIELs lacking inhibitory MHC-I receptors as mean ± SEM (n = 8). *Significant difference (P < .05; Mann-Whitney). (B) iIELs from B6 mice were incubated with anti-Ly49G2 mAb (4D11; ▨) or an isotype-matched control mAb (■). Unbound mAb was removed before stimulation with plate-bound anti-CD3 mAb. MIP-1α production by Ly49A or Ly49G2 single-positive iIELs is shown relative to iIELs lacking inhibitory MHC-I receptors as mean ± SEM (n = 4). *Significant difference (P < .05; Mann-Whitney). (C) iIELs from B6 mice were incubated with anti-Ly49E mAb (4D12; ) or an isotype-matched control mAb (■). Unbound mAb was removed before stimulation with plate-bound anti-CD3 mAb. MIP-1α production by Ly49E single-positive iIELs is shown relative to iIELs lacking inhibitory MHC-I receptors as mean ± SEM (n = 6). *Significant difference (P < .05; Mann-Whitney). (D) iIELs from B6 mice were incubated with anti-Ly49F mAb (HBF-719; ▨) or an isotype-matched control mAb (■). Unbound mAb was removed before stimulation with plate-bound anti-CD3 mAb. MIP-1α production by Ly49F single-positive iIELs is shown relative to iIELs lacking inhibitory MHC-I receptors as mean ± SEM (n = 3). There was no significant difference (NS; Mann-Whitney).