Growth factor effects on NUP98-HOXA10hd–induced HSC expansion in vitro. Graphic representation of the value of factor effects (β_i) divided by the model intercept (β₀) based on the prediction equation under “Factorial design experiments.” Three responses (Y) were modeled: the number of transduced cells of Lin⁻Sca-1⁺c-kit⁺CD43⁺Mac1^dim phenotype obtained in 7-day multifactorial expansion cultures (1) and the percentage of transduced cells detected 6 weeks (2) and 4 months (3) after transplantation in peripheral blood of recipients of 1/10 000 of each 7-day multifactorial expansion culture. Interaction effects are identified by the multiplication sign, ×. The factor effects are plotted with respect to growth factor concentrations scaled between −1 (low values) and 1 (high values). The values for each of the 4 factors per condition are presented in supplemental Table 7.