Sublytic erythrocytes are resistant to osmotic lysis. One microliter of fresh blood was added to 0.2 mL of a series of hypotonic solutions ranging from 0.1%-0.85% NaCl. The percentage of lysed erythrocytes was calculated based on absorbance. The degree of lysis in 0.1% and 0.85% NaCl was considered to be 100% and 0%, respectively. Data represent the means ± SEM.