Serological identification of HSP105 in B-NHL. (A) WB analysis of OFFGEL fractions from DOHH-2 proteins (F5, F6, and F7) probed with prevaccination (i) and postvaccination (ii) Igs from responder 14. (B) Silver staining of F5, F6, and F7 preparative SDS-PAGE for MS of the numbered protein bands (i) and WB analysis of F6 probed with polyclonal anti-HSP105 Ab (ii). (C) Immunoprecipitation (IP) of HSP105 from DOHH-2 whole cell lysate with polyclonal anti-HSP105 Ab, followed by WB analysis using pre- and postvaccination (Vax) Igs from responder 14, or mouse anti-HSP105 mAb as control. pI indicates isoelectric point.