Accumulation of glucosylceramide and glucosylsphingosine in cultured macrophages afterinactivation of glucocerebrosidase. Macrophages were differentiated from peripheral blood monocytes obtained from 2 different donors. After 7 days of culture, glucocerebrosidase was irreversibly inactivated for 4 days with an excess of the suicide inhibitor MDW941. Cells were harvested at indicated time points, and glucosylsphingosine (A) and glucosylceramide content (B) determined.