Figure 4
Figure 4. Segmental analysis of the newly defined promoter model. (A) Schematic illustration of the different parts of the CD11c/DC-STAMP promoter model and their respective location. pCD11c750-400bp, pCD11c750-574bp, and pCD11c500-400bp were cloned upstream of a SV40 minimal promoter into a lentiviral vector. (B) In vivo promoter analysis of the 3 pCD11c fragments indicated and the minimal SV40 promoter alone. Spleen cells from bone marrow chimeras were analyzed for GFP expression by flow cytometry. Histogram overlays of cells from C57BL/6 mice as negative controls are shown. Data are representative of 3 independently performed experiments (n = 4 or 5).

Segmental analysis of the newly defined promoter model. (A) Schematic illustration of the different parts of the CD11c/DC-STAMP promoter model and their respective location. pCD11c750-400bp, pCD11c750-574bp, and pCD11c500-400bp were cloned upstream of a SV40 minimal promoter into a lentiviral vector. (B) In vivo promoter analysis of the 3 pCD11c fragments indicated and the minimal SV40 promoter alone. Spleen cells from bone marrow chimeras were analyzed for GFP expression by flow cytometry. Histogram overlays of cells from C57BL/6 mice as negative controls are shown. Data are representative of 3 independently performed experiments (n = 4 or 5).

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