SET is overexpressed in CLL and NHL. (A) mRNA was isolated from 15 CLL patients and 6 normal B-cell samples, and SET mRNA was quantified by quantitative PCR. (B) Representative immunoblot of 2 normal B-cell extracts (N37 and N42) and 2 CLL cell extracts showing overexpression of both the α- and β-isoforms of SET as indicated in the CLL cells. Vertical lines have been inserted to indicate a repositioned gel lane. (C) SET/GAPDH protein ratios measured for 16 CLL patients and 6 normal B-cell samples used in panel A, showing a significant increase in expression of SET mRNA in B-CLL cells relative to normal B cells. (D) SET mRNA levels from 2 normal B-cell samples and Raji and Ramos cells quantified by quantitative PCR. ***P < .001 (E) Immunoblotting of 2 normal B-cell (N4 and N7) and Raji and Ramos cell extracts. Although SET levels appear higher in the normal samples, it is because of higher-intensity settings for this blot than the normals shown compared with the CLL samples in panel B. (F) Quantitation of the α- and β-isoforms of the SET protein (normalized to GAPDH) for Raji and Ramos NHL cell lines showing that the major increase in SET levels is because of increased α-isoform expression.