Cytotoxic activity and induction of apoptosis by celecoxib and E7123 in DLBCL lines. (A) Viability/concentration curves for celecoxib (black) or E7123 (gray) on DLBCL cell lines after 4-hour exposure. Error bars represent SE. (B) Apoptotic induction was evaluated by Hoechst staining after 24-hour exposure to 60μM celecoxib (CX) and 4-hour exposure to 60μM E7123 (original magnification ×400). Slides were viewed with an inverted microscope (Axiovert 200M). Images were acquired using a digital camera (Coolsnap; Photometrics) and processed with MetaMorph Version 5.01 software.