Resistance to IgE-mediated passive cutaneous anaphylaxis in mice with PigA-deficient mast cells. Mice were given injections with IgE anti-DNP in one ear and as control with medium in the other. On the next day, intravenous injections of DNP-BSA mixed with Evans Blue were given. Extravasation was visualized by blue staining of the ears (top panel). After extraction from the ears, extravasated Evans blue was quantified (bottom panel). Filled bars: VavCre littermate control mice; open bars: VavCrePigALoxP mice. Results represent mean ± SEM of 3 mice per group. Asterisk indicates significant difference (P < .01) with control mice; n.s. indicates not significant.