Binding affinity of WT and mutant EKLF for a number of EKLF-regulated genes. Saturation fluorescent gel shift assays were performed with recombinant WT, E325K, R331G, R328L, and K332Q EKLF ZF protein (0-2500nM) and 40nM Hex-labeled double-stranded oligonucleotides composed of EKLF recognition site and surrounding residues from the LU, CD44, ICAM4, AQP1, BCL11A, and p21 promoters (n = 3). Arrow indicates free Hex-labeled oligonucleotide. *Protein-DNA complexes. **Nonspecific binding or binding of EKLF to degenerate CACC sites.