Genes aberrantly expressed in InLu and CDA are directly regulated by EKLF. EKLF ChIP analysis was performed using day 10 ex vivo cultured human erythroblasts. Lane 1 indicates molecular weight marker; lane 2, total genomic DNA; lane 3, positive input control DNA; lane 4, DNA precipitated with EKLF antibody; lane 5, DNA precipitated with GFP (control) antibody; and lane 6, water control. DNA was analyzed by polymerase chain reaction using primers flanking the EKLF recognition sequence of the β-globin, LU, CD44, AQP1, ICAM4, p21, and SURF1 (control) promoters. As some samples were run in a different order on gels, lanes were cut and aligned for consistency.