Analysis of selected genes differentially expressed in sorted lymphoid leukemia samples. Semiquantitative RT-PCR analysis in 5 T (T4, Cd4⁺Cd8⁺; T5, Cd4⁺Cd8⁺; T6, Cd4⁺Cd8⁺; T7, Cd4⁺Cd8⁺; T8, Cd4⁺Cd8⁻) and 5 B (B4, Cd45⁺Cd19⁺Sca1⁺; B5, Cd45R⁺Cd19⁺Sca1⁺; B6, Cd45R⁺Cd19⁺Sca1⁺; B7, Cd45R⁺Cd19⁺Sca1⁺; B8, Cd45R⁺Cd19⁺Sca1⁺) leukemias. RT-PCRs were performed in triplicate for each gene. The actin gene was used as internal control in specific conditions described in “Semiquantitative RT-PCR” and expression level in each leukemia is presented as a selected gene/actin density ratio. (A) B leukemia-specific genes. (B) T leukemia-specific genes. (C) Genes common to both leukemias. Statistical analysis was performed using one-way analysis of variance, and P less than .05 was considered to be significant (*P < .05, **P < .01, ***P < .001) compared with the respective control (B cells from normal spleen [CB2] for the B leukemias and T cells from normal thymus [CT2] for the T leukemias).