American Society of Hematology

Figure 3

$Figure 3. Monocytes restore early CD40L expression. (A) Elutriated lymphocytes or (B) purified CD4+ T cells (> 95% pure) were plated alone or reconstituted with purified monocytes at the same density as in unfractionated PBMCs. Cultures were stimulated with anti-TCR and anti-CD28 mAb. Cells were harvested and stained for CD40L expression at the indicated times. Data are mean ± SE of 4 donors. (C) Elutriated lymphocytes were plated alone in the insert of a transwell dish (Lymphs) or reconstituted with purified monocytes (Lymphs + Monos) before plating. Alternatively, lymphocytes were plated in the insert, and monocytes were plated in the bottom of the well (Mono/Lymph). As a control, PBMCs were plated in the insert. Cells were stimulated with anti-TCR and anti-CD28 mAb, harvested, and stained for CD40L expression at the indicated times. Data are mean ± SE of 2 donors.$

Monocytes restore early CD40L expression. (A) Elutriated lymphocytes or (B) purified CD4⁺ T cells (> 95% pure) were plated alone or reconstituted with purified monocytes at the same density as in unfractionated PBMCs. Cultures were stimulated with anti-TCR and anti-CD28 mAb. Cells were harvested and stained for CD40L expression at the indicated times. Data are mean ± SE of 4 donors. (C) Elutriated lymphocytes were plated alone in the insert of a transwell dish (Lymphs) or reconstituted with purified monocytes (Lymphs + Monos) before plating. Alternatively, lymphocytes were plated in the insert, and monocytes were plated in the bottom of the well (Mono/Lymph). As a control, PBMCs were plated in the insert. Cells were stimulated with anti-TCR and anti-CD28 mAb, harvested, and stained for CD40L expression at the indicated times. Data are mean ± SE of 2 donors.

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