Figure 6
![Figure 6. Trafficking of CCR5 molecules internalized on chemokine or LTA treatment. Immunofluorescence analysis of CCR5 internalization and colocalization with endocytic molecules: monocytes preincubated on ice with MC-5 to label cell-surface CCR5 molecules were left in medium alone or treated with 100nM CCL5 or 10 μg/mL LTA for the indicated time to allow endocytosis. Fixed and permeabilized cells were costained for CCR5 (red), nucleus (blue), and endosomal markers [(A; green); early endosomes: EEA-1; early/recycling endosomes: TfR; late endosome/lysosomes: LAMP1], or the GPCR-specific adaptors for internalization β-arrestins (B; green). Single confocal sections are shown (scale bar = 5 μm).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/6/10.1182_blood-2010-05-287474/4/zh89991066310006.jpeg?Expires=1729896799&Signature=ZZXAbUqasBIY0eNAmb-9zk7MUq6RvNbpLeIgj3oivWPm~iLb7wukx70CAh~Up1AXZSw9G9gYBZ4y0~csfYjT0JhN8d0To3r7YT3-aiTjAM-NMjHe~N4Xux6euswSkA2lGl1XwV8yqUnFFUQ4PvEM0gsavaC7I-H9mpO-~YL12clcZy7MZWxLFxtlqQBkn0pFz-7ZwsiDC32pswyan1CikG8cozuvKM0lNxijw54z3VQPpDni~lwF0GmTSQw7qgAGOMb9agAwM0oiyzhtGTnewZs3c2cXJAFn3HpMME8Y2m6T9FVq8e-Z-e3qS2WhNO~ycJ1Og1~X1xTErfHxj2V54A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Trafficking of CCR5 molecules internalized on chemokine or LTA treatment. Immunofluorescence analysis of CCR5 internalization and colocalization with endocytic molecules: monocytes preincubated on ice with MC-5 to label cell-surface CCR5 molecules were left in medium alone or treated with 100nM CCL5 or 10 μg/mL LTA for the indicated time to allow endocytosis. Fixed and permeabilized cells were costained for CCR5 (red), nucleus (blue), and endosomal markers [(A; green); early endosomes: EEA-1; early/recycling endosomes: TfR; late endosome/lysosomes: LAMP1], or the GPCR-specific adaptors for internalization β-arrestins (B; green). Single confocal sections are shown (scale bar = 5 μm).
