Comparative descriptive and quantitative analysis of macrophage and neutrophil behavior after wounding. (A) Frames extracted from 11.5 hours of time lapse microscopy (supplemental Video 2) after tail transection in a Tg(mpeg1:Gal4-VP16/UAS:Kaede/mpx:EGFP) F1 embryo (red represents macrophages; and green, neutrophils). Bar represents 100 μm. (B-C) Cell migration paths for the first 6 neutrophils (B) and 6 macrophages (C) to arrive at the wound, followed for 18 hours after tail transection, extracted from a video of another wounded Tg(mpeg1:Gal4-VP16/UAS:Kaede/mpx:EGFP) F1 transgenic embryo. (D-H) Graphing of distance to wound edge for the same 6 neutrophils (D) and macrophages (E) as in panels B and C demonstrates an overall difference in migratory and dwelling behaviors. All macrophages migrate directly to the wound and remain near the wound edge for the remainder of the time course, whereas approximately 30% of neutrophils resume a roaming behavior from 3 to 6 hours after wounding. Insets i and ii for each panel present collected x/y movement vectors before (i) and after (ii) wound arrival. Strong directionality is demonstrated by both cell types before arrival, but macrophages move with a slower prearrival velocity (F) and with a stronger directionality reflected by their higher meandering index (direct path length/actual path length) (G). In-wound persistence index, the percentage of remaining time spent at the wound edge after arrival (H), demonstrates the propensity of macrophages to remain in the wound margin for long periods after their arrival, whereas neutrophils show a significantly larger spread of behaviors. Descriptive statistics: (B-E) Data for the same 6 cells of each type. (F-H) Data points are individual cells from 4 embryos imaged for 18 hours after wounding. (F,G) Bars represent mean plus or minus SD. Tests of significance: (F,G) t test, 2-tailed. (H) Mann-Whitney test, 2-tailed.