miR-181b expression values significantly decrease in progressive but not in stable CLL over time (training set). Relative expression of the mature miR-181b in the first time point (violet blocks) and last time point (yellow blocks) from sequential samples of patients with CLL with either progressive (A) or stable (B) disease. The expression has been determined by stem-loop real-time qPCR. Each sample of data was normalized to the endogenous reference RNU44 with the use of the 2^−Δct method. P value is the result of the paired t test on log10-transformed values. Error bars indicate SD (mean ± SD), n = 3.