5-HETE-PLs are formed during acute bacterial peritonitis in mice and humans. (A-C) Formation of 5-HETE-PLs in murine live bacterial peritonitis. S epidermidis (5 × 108 cfu/mouse) was administered intraperitoneally, and lavage was harvested 3, 6, 12, or 18 hours later (n = 4 mice; mean ± SEM). Lipids were extracted and analyzed with LC/MS/MS (A) Time course of formation of 5-HETE-PLs. (B) Time course of formation of free 5-HETE. (C) Time course of cellular infiltration, neutrophils (Nϕ), macrophages (Mϕ), and lymphocytes (Lympho) n = 4; *P < .05, **P < .01, and **P < .001 versus phosphate-buffered saline (PBS). (D) 5-HETE-PLs were elevated in patients with confirmed bacterial infection but not in lavage from patients who were culture negative or from stable control patients. Lipids were extracted and analyzed with LC/MS/MS The numbers to the right of the bars indicate the number of infiltrating neutrophils per lavage. Hs indicates Hemolytic streptococcus; C, Corynebacterium; Pv, Proteus vulgaris; K, Klebsiella and Ec, Escherichia coli.
