klf2a expression is reduced in tnnt2a morphants, but maintained in moonshine mutants. (A-C) WISH showed that klf2a expression in the trunk region was reduced in the tnnt2aMO-injected embryos, but maintained in moonshine mutants. (D) Quantitative RT-PCR analysis of klf2a expression in the dissected trunk region of control and tnnt2aMO embryos at 24 and 36 hpf, respectively (mean ± SD, t test, *P < .05, n = 3). (E) Western blot showed Klf2a protein was significantly down-regulated in tnnt2a and klf2aMO-injected embryos.