qRT-PCR analysis of hepatic gene expression. The expression of several genes was measured in individual rats from the 6 dietary treatment groups. Shown are the 2 genes that showed significant changes in expression among the groups (A,C) and the data for Cp (which showed no significant differences; B). In all panels, bars with different letters atop or beneath error bars are statistically different from one other (P < .05). Hamp, hepcidin; Ankrd37, ankyrin repeat domain protein 37. n = 7 for CuD group and n = 8 for all other groups. Data are expressed as means ± SD except for data in panel C, which are expressed as means ± SEM (because a log scale is used).