Iron and copper levels in rat liver and serum. Means ± SD are shown. Liver samples were dried and wet-ashed before inductively coupled plasma-mass spectrometry analysis; data are presented as micrograms per gram dry weight. Serum samples were diluted and analyzed similarly; data are presented as micrograms per milliliter for copper or micrograms per deciliter for iron. Bars with different letters on top are statistically different from one another (P < .05; n = 7-9/group).