mTORC1 and mTORC2 complexes are present in human platelets. (A) Centrifugally cleared lysates-(50 μg) from human platelets or MCF10A breast epithelial cells were analyzed by Western blot for levels of mTOR, Raptor, Rictor, or GβL. (B) mTORC1 complexes were identified by immunoprecipitating Raptor from platelet lysates and Western blotting for associated mTOR. (C) mTORC2 complexes were identified by immunoprecipitation of Rictor followed by Western blotting for coprecipitating mTOR. Nonspecific immunoglobulins (IgG) served as a negative control for immunoprecipitations. Immunoprecipitation results are representative of 3 independent experiments.