CCR7 is involved in the in vivo migration of neutrophils to the draining lymph nodes. (A) The expression of CCR7 on WT mouse neutrophils, stimulated or not with 50 ng/mL of GM-CSF and/or 20 ng/mL of IL-17, was evaluated by FACS using anti-CCR7 mAb (white histograms) compared with a control mAb (gray histograms). Results are representative of 1 of 5 experiments. (B) Neutrophils isolated from WT and Ccr7^−/− mice were labeled with PKH26 and activated with GM-CSF and IL-17 before injection in WT mice. Lymph nodes cells were collected 6 hours after injection and incubated with an anti-neutrophil mAb. Results are expressed as the number of PKH26-labeled neutrophils in the draining lymph nodes (mean ± SEM, n = 5). *P < .05.