Role of CCR7 in the in vivo migration of neutrophils. (A) The density of neutrophils in blood and bone marrow from WT and Ccr7^−/− mice was determined by FACS. Results are expressed as density of neutrophils (mean ± SEM, n = 3). (B) WT and Ccr7^−/− mice were injected intradermally with PBS or CFA. The presence of neutrophils in the draining lymph nodes was analyzed 6 hours after injection by FACS using PE-labeled anti-Ly6G and FITC-labeled anti-neutrophil mAbs. Left, results are expressed as the number of neutrophils in the draining lymph nodes (mean ± SEM, n = 5). *P < .05. Right, dot-plot representative of 1 of 5 experiments.