Figure 3
CCR7 is not expressed at the surface of apoptotic neutrophils. (A) Human neutrophils were cultured for 72 hours in RPMI-1640 medium containing 1% FCS before analysis of CCR5 and CCR7 expression. Neutrophil apoptosis was determined by annexin V and 7-AAD staining and analyzed by cytometry. Histograms show membrane CCR5 and CCR7 expression by live (annexin V− 7-AAD−; R1), early apoptotic (annexin V+ 7-AAD−; R2), and late apoptotic (annexin V+ 7-AAD+; R3) neutrophils. Results are representative of 1 of 5 independent experiments. (B) Analysis of CCR7 and CCR5 expression by live (■) and apoptotic (□) human neutrophils. Results are expressed in mean fluorescence intensity values (mean ± SEM, n = 5). *P < .05. PMN indicates polymorphonuclear neutrophil.

CCR7 is not expressed at the surface of apoptotic neutrophils. (A) Human neutrophils were cultured for 72 hours in RPMI-1640 medium containing 1% FCS before analysis of CCR5 and CCR7 expression. Neutrophil apoptosis was determined by annexin V and 7-AAD staining and analyzed by cytometry. Histograms show membrane CCR5 and CCR7 expression by live (annexin V− 7-AAD−; R1), early apoptotic (annexin V+ 7-AAD−; R2), and late apoptotic (annexin V+ 7-AAD+; R3) neutrophils. Results are representative of 1 of 5 independent experiments. (B) Analysis of CCR7 and CCR5 expression by live (■) and apoptotic (□) human neutrophils. Results are expressed in mean fluorescence intensity values (mean ± SEM, n = 5). *P < .05. PMN indicates polymorphonuclear neutrophil.

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