Localization of CCR7 in neutrophils by confocal microscopy. (A) Confocal microscopy analysis of CCR7 in freshly isolated human neutrophils. Cells were not permeabilized (−; left panels) or permeabilized (+; right panels) and stained for CCR7 and MPO, CCR7 and gelatinase, or CCR7 and lactoferrin. Nuclei were stained with DAPI. (B) Neutrophils were fixed and stained for CCR7 (green) and CXCR2 (red) expression. Nuclei were stained with DAPI.