CD1d expression and glycolipid antigen presentation in Elf-1^−/−mice are normal. (A) Representative histograms of CD1d expression on thymocytes and thymic DCs isolated from WT and Elf-1^−/− mice. Cells were stained with either a control mAb (open histogram) or anti–CD1d (filled histogram) and analyzed by flow cytometry. (B) The iNKT-cell hybridomas DN3.A4 and N38-2C12 were cocultured with either WT or Elf-1^−/− thymocytes in the absence (left) or presence (right) of αGalCer. After 24 hours, IL-2 levels in the supernatant were detected by ELISA. Error bars represent the SD of triplicate wells. Results are representative of 2 experiments. (C) iNKT cells were enriched from Vα14Tg mice and cocultured with either WT or Elf-1^−/− DCs in the absence or presence of αGalCer. IFN-γ levels in the supernatant were detected by ELISA. Error bars represent the SD of triplicate wells. Results are representative of 2 experiments.