Figure 3
Figure 3. HIF binding regulates gene expression over long genomic intervals. Genomic binding of HIF-1α, HIF-2α, and HIF-1β subunits at the (A) ERRFI1 and (B) ARRDC3 gene loci. The number of reads per 100-bp window and the location of each gene are shown. (C) ChIP–quantitative PCR confirmation of the ChIP-seq defined binding peaks. (D) Expression-quantitative PCR confirmation of gene regulation in response to 16 hours at 0.5% hypoxia. (E) ChIP–quantitative PCR demonstrating RNA Pol2 recruitment to the ERRFI1 and ARRDC3 HIF-binding sites in normoxia and after 16 hours at 0.5% hypoxia. (F) Luciferase reporter assay showing activity of a heterologous luciferase reporter gene fused to sequences spanning each HIF-binding site in normoxia and after 16 hours at 0.5% hypoxia.

HIF binding regulates gene expression over long genomic intervals. Genomic binding of HIF-1α, HIF-2α, and HIF-1β subunits at the (A) ERRFI1 and (B) ARRDC3 gene loci. The number of reads per 100-bp window and the location of each gene are shown. (C) ChIP–quantitative PCR confirmation of the ChIP-seq defined binding peaks. (D) Expression-quantitative PCR confirmation of gene regulation in response to 16 hours at 0.5% hypoxia. (E) ChIP–quantitative PCR demonstrating RNA Pol2 recruitment to the ERRFI1 and ARRDC3 HIF-binding sites in normoxia and after 16 hours at 0.5% hypoxia. (F) Luciferase reporter assay showing activity of a heterologous luciferase reporter gene fused to sequences spanning each HIF-binding site in normoxia and after 16 hours at 0.5% hypoxia.

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