Nystatin increases the inhibitory effects of endostatin on endothelial cell activities. (A) HUVECs were treated with 0 or 25 μg/mL nystatin, incubated with 0 or 10 μg/mL endostatin for 30 minutes, and then stimulated with 10 ng/mL VEGF 165 for 10 minutes. Phosphorylation levels and basal protein levels of p38 MAPK and ERK1/2 were assessed by immunoblotting. (B-E) HUVECs were treated with saline, 25 μg/mL nystatin, 10 μg/mL endostatin, or a combination of nystatin and endostatin, then measured for tube formation at 6 hours (B-C), transwell migration at 4 hours (D), and the percentage of scratch wound healing at 12 hours (E), respectively. Images from 5 random fields per well of 3 independent experiments were obtained by an optical microscope. ***P < .001. Error bars represent SDs.