TF expression was up-regulated in apoER2^+/+ mice treated with IgG-APS or dimer through an interaction with ApoER2. ApoER2^+/+ (filled bars) or ApoER2^−/− (open bars) mice were injected either with IgG-NHS, IgG-APS, monomer, dimer, PBS, or LPS as described in the section “Ex vivo immunolabeling of TF in harvested murine peritoneal macrophages.” TF expression was determined by immunostaining with specific Qdot bioconjugates and quantitated after examination with a dual-photon laser confocal microscope. Mice treated with LPS or PBS were used as controls. (A) Representative images of TF expression of the different treatment groups. The red fluorescent staining (655-nm Qdot bioconjugate) indicates surface TF immunoreactivity, whereas the blue color represents cell nuclei stained with Hoechst dye. (B) Quantitative TF expression are assessed as fluorescence intensity in AU (mean ± SD; n = 10 images per mouse and 2 mice per group). †Statistically different from PBS-treated mice control (P < .0001). ¶Statistically different from apoER2^+/+ treated with control, NHS (P < .0001), or monomer (P < .0001). *Statistically different from apoER2^+/+ treated with agonist, IgG-APS (P < .0001), or dimer (P < .0001).