Figure 4
ADAM10 regulates CCND1 expression in MCL. (A) Western blots showing down-regulation of CCND1 and up-regulation of p21Waf1 in Jeko-1 and Mino cells after ADAM10 down-regulation using siRNA. (B) qRT-PCR showing down-regulation of the CCND1 mRNA in Jeko-1 and Mino cells 24 hours after down-regulation of ADAM10 using siRNA. (C) Western blots showing up-regulation of the nuclear level of CCND1 protein in SP53 cells 24 hours after the addition of human recombinant ADAM10 (100 ng/mL); histone deacetylase was used as the loading control for the nuclear extract.
