ADAM10 promotes cell growth in MCL. (A) Western blots showing down-regulation of the precursor and active/mature form of ADAM10 in Jeko-1 and Mino cells with the use of siRNA. (B) ADAM10 knockdown induced significant inhibition of cell growth in Mino and Jeko-1 cells 3 days after transfection, as assessed by the trypan blue exclusion assay (P = .01 and P = .007, respectively). Triplicate experiments were performed. (C) ADAM10 knockdown induced significant inhibition of cell growth in Mino and Jeko-1 cells 3 days after transfection, as assessed by MTS assay (P < .0001 and P = .01, respectively). Triplicate experiments were performed. (D) Addition of human recombinant ADAM10 (100 ng/mL) to SP53 and Mino cells induced a significant increase in their growth at 24 hours (P = .008 and P = .01, respectively).