ELISA. Solid-phase reagents PANC1 cell lysate, HEK293T lysate, insulin, ssDNA, lipopolysaccharide (LPS), TG, BSA, and HSA are given in the x-axis, respectively, whereas light absorption is indicated in the y-axis. (A) Reaction of control buffer and 10 μg/mL of the 4 different antibodies Ab10, Ab25, Ab129, and Ab152, respectively, with the solid-phase reagents. All antibodies, except Ab152 and control buffer, react with the solid phase reagents. (B) Reaction of Ab25 and a mouse monoclonal Ab149, anti-proGRP, with the solid-phase reagents using different buffers. Ab25, but not Ab149, reacts with the reagents. Ab149 reacts solely with the cell lysates, as expected. The addition of Tween20 inhibits the reaction of Ab25 with HSA, BSA and reduces the reaction with insulin and galactosidase. Ab25 reacts in a dose-dependent fashion with the solid phase reagents (panel C) and its binding to TG can be blocked by TG and heterologous binding reagents ssDNA and galactosidase (panel D).