Reactivity of the recombinant antibodies in diverse tissues and cells. The antibodies stain cytoplasmic autoantigens in a diverse array of cell types (immunoperoxidase staining): (A) colonic epithelial cells in the appendix (mAb 25; magnification ×200); (B) tubular epithelial cells in the kidney (mAb 129; magnification ×100); (C) respiratory epithelial cells in the bronchus (mAb 10; ×400); (D) pancreatic ductal epithelial cells as well as exocrine secretory cells (mAb 129; ×200); (E) ductal epithelial cells in the salivary gland (mAb 25; ×200); (F) acinar cells in the stomach (mAb 10; ×100); (G) SMZL cells (mAb 10; ×400); (H) red cell precursor cells in the bone marrow (mAb 10; ×400); (I) marginal zone B lymphocytes in the spleen (mAb 129; ×100); (J) a subpopulation of monocytoid B cells in the lymph node (mAb 129; ×100);(K) tingible body macrophages in germinal centers from a lymph node (mAb 25; ×400); (L) cells of pancreatic carcinoma cell line PANC1 (mAb 25; ×400).