Induction of transient hyperphosphorylation of paratarg-7 in T-cells from healthy donors carrying wild-type paratarg-7. T cells were stimulated with anti-CD3 and anti-CD28 for 48 hours. IEF analysis followed by immunodetection of paratarg-7 was done after the indicated days of incubation (d0-d21). Unstimulated T-cell lysates from a patient carrying hyperphosphorylated paratarg-7 (lane a) or from a healthy donor carrying wild-type paratarg-7 (lane b) were used as control. Hyperphosphorylated paratarg-7 becomes detectable on day 4 and persists until day 18.