Demonstration of the trimeric complex of paratarg-7/PKCζ/PP2A by coimmunoprecipitation. Recombinant FLAG-tagged paratarg-7 expressed in HEK293 cells was precipitated using FLAG Abs (0.5 μg) and protein-G. The precipitate was subjected to PAGE followed by immunodetection with anti-FLAG (lanes a-b), anti-PP2A subunit C (lanes c-d) or anti-PKCζ (lanes e-f). As a negative control similarly expressed FLAG-tagged recombinant SMCHD1 protein was used. Lanes a, c, and e: control.