GAL1 is expressed by BM stromal cells. (A) BM sections of wild-type mice were stained with anti-GAL1, anti-CD45, and Sytox Blue (nuclei) (original magnification ×40). Bar represents 10 μm. (B-C) Representative regions of BM sections stained as in panel A (original magnification 63×/1.4 NA oil objective). Hematopoietic cells (arrow), reticular cells (arrowhead), and osteoblasts (ob) expressing GAL1 are designated. Bar represents 20 μm. (D) BM sections of stroma-GFP mice were stained with anti-GFP and anti-GAL1 antibodies (original magnification 40×/1.3 NA oil objective). *Characteristic cuboid or fibroblatic-like GAL1⁺ stromal cells are indicated. Bar represents 10 μm. Hatches boxes indicate the areas magnified in lower panels. GAL1⁺ (E) and GAL1⁻ (F) stromal cells are shown. Bar represents 20 μm. Images acquired on Zeiss LSM510 Meta and analyzed with ImageJ 1.44.