GAL1 expression in BM cells. Different BM subpopulations were analyzed for intracellular expression of GAL1 by flow cytometry. GAL1 intracellular staining in C57Bl/6 mice (unshaded) was compared with GAL1^−/− mice as a negative control (shaded). The gating strategy is shown in the figure, and the cellular subsets were defined as follows: (A) Pro-B/pre-B (CD19⁺B220⁺IgM⁻IgD⁻), immature B (CD19⁺B220⁺IgM⁺IgD⁻), and recirculating B cells (CD19⁺B220⁺IgM⁺IgD⁺). (B) CD117⁺ hematopoietic progenitors (Ter119⁻CD19⁻B220⁻CD3⁻Gr1⁻CD117⁺). (C) Immature myeloid cells (CD11b⁺Gr1⁺), granulocytes (CD11b^hiGr1^hi). (D) NK (NK1.1⁺CD3⁻), NKT (NK1.1⁺CD3⁺), and T cells (NK1.1⁻CD3⁺). (E) Erythrocytes (Ter119⁺). (F) Plasmacytoid dendritic cells (CD11c⁺B220⁺). (G) Lymphoid DC (CD11c⁺CD11b⁻) and myeloid DC (CD11c⁺CD11b⁺). (H) BM stromal cells (CD45⁻Lin⁻). The lineage (Lin) staining corresponds to a mix of Ter119, CD19, CD11c, CD117, and Gr1.