Flow cytometric analysis of phenotypic DC maturation and RAGE expression on immature iDCs stimulated with G-β₂GPI. (A) Flow cytometric analysis of phenotypic DC maturation. After 18 hours of incubation, G-β₂GPI and M-β₂GPI induced similar DC maturation, whereas G-β₂GPI only induced statistically significant up-regulation of RAGE. For HLA-DR: *P < .01 and ^†P < .001; for CD83 and CD86: *P < .001, †P < .01, and ^‡P < .01 comparing G-β₂GPI vs α-RAGE + G-β₂GPI; for CD80: *P < .05 and ^†P < .01; for CD40: *P < .001, ^†P < .05, and ^‡P < .05 comparing G-β₂GPI vs α-RAGE + G-β₂GPI; for RAGE: *P < .001 and ^†P < .001 comparing G-β₂GPI vs α-RAGE + G-β₂GPI. (B) Flow cytometric analysis of RAGE expression on iDCs stimulated with G-β₂GPI at different time points. G-β₂GPI induced statistically significant up-regulation of RAGE expression that remained elevated until 72 hours. *P < .001 and ^†P < .05.