Relation between results of MRD monitoring with the new markers and those of standard MRD assays. (A) Comparison with flow cytometry using standard markers. The top 16 differentially expressed markers listed in Table 2 were included for a total of 258 tests (number of tests for each marker: CD44, 15; BCL2, 18; HSPB1, 32; CD73, 15; CD24, 7; CD123, 34; CD72, 10; CD86, 21; CD200, 29; CD79b, 5; CD164, 7; CD304, 12; CD97, 20; CD102, 10; CD99, 8; CD300A, 15). Spearman regression analysis of positive MRD results by both methods: r = 0.9816, P < .0001). (B) Comparison with PCR amplification of Ag-receptor genes performed in a subset of the samples (r = 0.8178, P < .0001). Each symbol represents results obtained by 4-color flow cytometry including Abs against CD19, CD10, CD34 and one of the new markers. The dashed line is the line of identity.