DCs matured with LPS/R848 agonists displaying reduced expression of CD83, CD80, and/or CD86 prime a response with reduced ability to produce effector cytokines. CD8⁺ T cells primed by the indicated DC subpopulations were restimulated with 10 μg/mL of Melan-A_26-35 peptide 9 days after priming, and intracellular IL2, IFNγ, and TNF production were analyzed simultaneously. Bars indicate the mean proportion of total responding CD8⁺ T cells producing the cytokines indicated (± SEM). Data are combined from 2 donors generated in independent experiments (A) and representative of 2 independent sorting experiments (B). However, for the DC-D phenotype, the limited number of DCs generated after cytokine maturation permitted establishment of only one priming well, so SEM could not be calculated for this population.