Increased myeloid and decreased lymphoid output in PB (A-D) and BM (E-H) of Crebbp+/− recipients. (A) Average frequency ± SEM of donor-derived, wild-type (Wt) granulocytes based on scatter profiles of PB cells in Wt (●) and Crebbp+/− recipients (○) (n ≥ 12; *P < .001). (B-H) Data obtained at 5 months after transplantation. (B) Representative FACS profiles of donor-derived (Gr-1hiCD11b+) granulocytes. (C) Average percentage of granulocytes (G), lymphocytes (L), and other cells (O). (D) Representative FACS profiles of donor-derived (B220+) B cells and (CD4/8+) T cells. (E) Data represent the average number ± SEM of nucleated BM cells obtained from 2 femurs (first 2 bars) and weight (last 2 bars) of Wt and Crebbp+/− recipients (n ≥ 11; *P < 10−8). (F) Representative FACS profiles of donor-derived Gr-1loCD11b+ (left gate) and Gr-1hiCD11b+ myeloid cells (right gate). (G) Average frequency ± SEM of donor-derived Gr-1loCD11b+ and Gr-1hi CD11b+ myeloid cells and B220+ cells (n ≥ 7; *P < .002). (H) Average frequency ± SEM of donor-derived granulocyte-macrophage progenitors (GMPs), megakaryocyte-erythroid progenitors (MEPs), common myeloid progenitors (CMPs) and common lymphoid progenitors (CLPs; n ≥ 12; *P < .05).
